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Thiamine works as a co-enzyme for complexes including enzymes essential for intracellular glucose metabolism, such as transketolase, pyruvate dehydrogenase (PDH), and -ketoglutarate dehydrogenase

Lash GE, Schiessl B, Kirkley M, et al

Yuan J, Luo RZ, Fujii S, Wang L, Hu W, Andreeff M , Pan Y, Kadota M, Oshimura M, Sahin AA, Issa JP, Bast RC, Jr, Yu Y

Therefore, on column tag removal is considered more effective for large scale purifications because it allows for better protein recovery and also reuses protease and columns

Drugs and Biologicals with Average Annual Total Cost Under Part D Below the Applicable Threshold Under section 1860D-14B(g)(1)(B) of the Act, a drug or biological is excluded from the definition of a Part D rebatable drug if the average annual total cost under Part D for such period per individual who uses such a drug or biological product is less than $100 per year, as determined by the Secretary using the most recent data available, or, if data are not available, as estimated by the Secretary