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The following primary antibodies (all from Cell Signaling Technology, Danvers, MA, USA) were used: anti-P-m-TOR (1:1000 dilution, Cat number #5536), anti-m-TOR (1:1000 dilution, Cat number #2983), anti-Beta Actin (1:1000 dilution, Cat number #3700), anti-cleaved PARP (1:2000, Cat number #94885), anti-cleaved caspase 3 (1:1000, Cat number #9661), anti-p62 (1:1000, #88588), anti LC3 (1:1000, Cell signaling technology Cat number #3868) for overnight at 4 C

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So, while vegetarians may be able to intake sufficient quantities through eggs, cheese, and yogurt, vegans must find other B12 supplements to stay healthy

Invitrogen SuperScript IV VILO Master Mix (Thermo Fisher Scientific) was employed to convert 250 ng of total RNA into cDNA as per the manufacturers instructions, including prior genomic DNA removal with kit-supplied ezDNase