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H.KeimJ.et al (2021)

Most researchers prefer the 20mg/ml standard because it balances precision with practicality

For ultravioletvisible measurements, one-step in vitro reactions with the standard reaction conditions as defined above were performed in a total volume of 1.2 ml in 2 ml microcentrifuge tubes incubated at 30 C for 15 min at 1,200 rpm using a 3 mm shaking diameter

Marks JG, Belsito DV, DeLeo VA, et al

Fluorescence imaging was performed under a confocal microscope (Zeiss LSM 810, Germany or Nikon AXR, Japan), and immunohistochemistry imaging was performed under a microscope (Nikon Eclipse SI)

Native-like SARS-CoV-2 spike glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine ACS Central Science2021Watanabe, Y