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Do not spray the water directly onto the lyophilized powder cake

This creates a cystine/cysteine redox cycle across the membrane and creates a reducing extracellular environment that supports cancer cell growth and survival ( Cell Death Ferroptosis is an iron-dependent cell death which is accompanied by iron accumulation and lipid peroxidation

Highland, Carolyn M

Surface nanostructuring amplifies contact-killing through enhanced reactivity and mechanical disruption (Acharya et al., 2021

Prior to Reconstitution: Keep the lyophilized vial inside a dark, moisture-free environment

Then 10 6 cells of each of the chosen cell lines were seeded in standard culture plates of 96 wells and kept in an incubator with atmospheric conditions of 95% humidity and 5% carbon dioxide