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This may be related to sequestration of CoA within mitochondria, depletion of mitochondrial DNA, increased production of ROS, and alterations in gene expression [2]

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The molecular pathologic processes believed to occur in smallpox take a toll on GSH concentrations: (a) induction of iNOS with increased NO production, (b) the generation of superoxide, (c) formation of peroxynitrite, (d) GSH-consuming chain reactions of ROS and RNS, (e) TNFa related oxidative stress, the induction of Cox-2 with increased oxidation of arachidonic acid, (g) oxidative bursts from macrophages engaged in phagocytosis at sites of infection and inflammation, and related cascades

Identification of microbial signatures offers promise for novel diagnostic and therapeutic strategies

STEP 1 : In part one of this process, the enzyme glutamyl cysteine synthetase uses glutamic acid, cysteine, adenosine triphosphate or ATP (energy), and magnesium to form gamma-glutamylcysteine