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FoxO transcription factors and stem cell homeostasis: insights from the hematopoietic system

Lysosomal inhibitor Baf-A1 and autophagy inhibitor 3-MA also protected against cell death induced by GSH depletion, highlighting the role of autophagy in ferroptosis

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Galovic et al., 2021)

The membranes were blocked with 5% milk in 0.1% Tween-20/TBS (Tris-buffered saline) for 1 h at room temperature and they were incubated with rabbit monoclonal anti-phospho-H2AX (Ser139) antibody (#9718, Cell Signaling Technology, Danvers, MA, USA) diluted 1:1000 in 5% milk and 5% BSA in 0.1% TBST or anti-phospho-p53 (Ser15) (#9284, CST) diluted 1:1000 in 5% milk in 0.1% TBST, or anti-p21 (#2947, CST) or anti-p53 (#2527, CST) diluted 1:1000 in 5% BSA in 0.1% TBST overnight at 4 C