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Another appraoch would be to compare the brain concentration profiles of heroin and 6-MAM with the time-course of early neurobiological effects

The second assay was performed as follows: PBMCs cultured in 0.5 ml RPMI (Gibco) +10% Human AB serum (Sigma) was pulsed with 5 M of synthetically synthesized peptides (JPT)

doi: 10.1007/978-1-4613-1083-9_4

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Deficiencies of VIC can lead to neuropsychiatric disorders, and many studies are trying to highlight its effect as an adjuvant therapy in this regard [106,107]