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this step eliminated external contaminants (blood, etc.)

GriffinGHewisonMHopkinJKennyRQuintonRRhodesJet alPerspective: vitamin D supplementation prevents rickets and acute respiratory infections when given as daily maintenance but not as intermittent bolus: implications for COVID-19

doi: 10.1038/s41598-022-20658-x 277 TuCTaoFQinYWuMChengJXieMet al

prausnitzii in feces, suggesting its potential regulatory role in intestinal immunity 81

Recommended Containers Avoid: Standard polypropylene tubes without protein low-bind treatment (significant adsorption at ng/mL concentrations) Polystyrene containers (higher adsorption than polypropylene) Standard glass without silanization At concentrations of 10100 ng/mL, even modest adsorption to tube walls can meaningfully reduce the effective concentration and introduce variability between experiments

*Mutations of these genes may lead to increased sensitivity to growth factors with subsequent uncontrollable cellular division and cancerogenesis