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After infection for 48 h, cells were sub-seeded into 96-well plates and selected for single colonies using puromycin (2 g ml 1 ) for 1 week

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The researchers utilized advanced mass spectrometry and RNA-sequencing to map the exact metabolic dysfunctions driving these conditions, uncovering striking evidence of severe oxidative stress and mitochondrial lipid oxidative damage mediated by glutathione peroxidase 4, as documented in the PNAS metabolic study

These include N,N -bis(2-hydroxybenzyl)ethylenediamine- N,N -diacetic acid (HBED), the hydrazones pyridoxal isonicotinoyl hydrazone (PIH) and salicylaldehyde isonicotinoyl hydrazone (SIH), desferrithiocin, and, 2,3-dihydrobenzoic acid

For liver-specific genetic deletions, mice were injected via the tail vein with 2.510 11 GC of AAV-TBG-Cre (Addgene, 107787-AAV8) in PBS