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In all cases, the experiments were done by triplicate, and data represented as percentage of the fluorescence obtained with the untreated samples of the corresponding cell line

GPx6 is a tetramer containing selenocysteine in the active center (Brigelius-Floh and Floh 2020)

The mass spectrum in positive ionization mode for complex 2 after 1 min of incubation showed three signals, one at m/z = 613 corresponding to the GSSG and two signals of an iron complex at m/z = 254 and at m/z = 376, corresponding to the [M L] + and [M + Na] + species

doi:10.5501/wjv.v10.i2.34

Iron supply primarily depends on dietary intake ( 2+ , while transferrin in portal blood only binds Fe 3+ ), leading to its degradation in enterocytes and macrophages, resulting in intracellular iron retention and reduced circulating iron levels

The fact that FONE70 alone has induced an excessive amount of ROS in cells and possessed potent cellular destructive power indicates the involvement of endogenous catalase