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The PCR programme used consisted of a first denaturation step at 98 C for 3 min followed by 20 cycles of: (a) 1 min at 98 C, (b) 45 s at 56 C, and (c) 7 min at 72 C

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Multi-draw: if using BAC water in a truly sterile vial with regular aseptic access, you might extend use to e.g., 1430 days (depending on peptide)

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