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In such cases, cytoplasmic cPla 2 fluorescence became an inverse cell lysis marker, whose loss mirrored the rise of the Sytox blue signal (Extended Data Fig

Transplantation of mSOD1 G93A microglia into PU.1 / mice, which lack macrophages, neutrophils, T- and B-lymphocytes, and microglia, did not induce motor neuron degeneration, confirming that mSOD1 in microglia alone is not sufficient to initiate disease ([17, 18, 35]

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Samples were then centrifuged at 15,000 g for 12 min and 20 l of the supernatant was added to 200 l of -mercaptoethanol (500 mM)

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Besides, compared the ergothioneine yield of strains in PSB-HSs and PSB-HSl (contained 3 mM histidine and SAM in PSB), they showed that the excessive substrates may decrease the yield of ergothioneine, which suggested that the production of ergothioneine could be further optimized by starting from the adjustment of the concentration of additives