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The nanomaterial suspension was ultrasonicated for 30 min in the dark, after which zeta potential, UV-visible absorption spectra, and particle size distribution were calculated using a Xetasizer Nano ZS90 (Malvern Instruments, Marburg, UK) [6]

In order to test the relevance of this phosphorylation to RBOHD-mediated ROS production, we measured the transient ROS burst by modification of a previously described procedure 42