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For GSSG determination, the cell extracts were treated with 4-vinyl pyridine (Sigma-Aldrich) for GSH derivatization, and the excess of 4-vinyl pyridine was neutralized with triethanolamine

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Image 1 - the transsulfuration pathway linked to the methionine cycle via homocysteine is important for glutathione production

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After 72 h coculture, incubation with M1 macrophages did not modulate the differentiation of LR-MSCs