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In the first study, 32 patients with refractory IC/BPS were given intravesical instillation of heparin-lidocaine (20,000 units heparin, 5 mL 4% alkalized lidocaine, 25 mL 7% sodium bicarbonate) weekly for 12 weeks

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The samples were then rinsed with ice-cold phosphate buffer (0.9 mL saline, pH 7.0 per 0.1 g tissue), and centrifuged at 3000 g for 10 min