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Biliverdin reductase A attenuates hepatic steatosis by inhibition of glycogen synthase kinase (GSK) 3 Phosphorylation of serine 73 of peroxisome proliferator-activated receptor (PPAR)

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Trx-mGH inclusion body pellets (from equal volumes of culture medium) were resuspended in equal volumes of resuspension buffer (50 mM TrisHCl, 2 M urea, pH 10.5) and were subjected to either one or three cycles of freezethaw at 20 C or solubilised at 4 C overnight (Fig

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