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Step 3: Push the plunger in to add 10 mL of air to the vial and then draw out 10 mL of bacteriostatic water by slowly pulling the plunger back out

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This work used the PBRC facilities of the Transgenic, Comparative Biology, Genomics Core and Clinical Research Laboratory supported in part by COBRE (NIH 8P20GM103528) and the NORC (NIH 2P30-DK072476-11A1) center grants from the National Institutes of Health

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For enhanced nutrient synergy, pair antioxidants like vitamins E and C to protect neuronal tissue and support synaptic communication

Check for Air Bubbles : Tap the syringe gently to move any air bubbles to the top, then push the plunger slightly to expel the air