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Glycolytic intermediate 3-PG is converted to serine through a three-step enzymatic cascade, generating metabolite intermediates for lipid, nucleic acid, protein, and antioxidant biosynthesis

Particularly, in SF3B1-MDS, there is a significant decrease in lymphocyte percentages and an increase in erythroid percentages

Confocal images were taken using an LSM-510 META laser scanning confocal microscope (Zeiss, Germany) and ZEN 2012 imaging software (Zeiss) or BZ-X700 fluorescence microscope (Keyence, Japan) available through the Fluorescence Imaging Core of the Hopkins Basic Research Digestive Disease Development Center

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doi: 10.1155/2022/4024331 11 PainuliSQuispeCHerrera-BravoJSemwalPMartorellMAlmarhoonZMet al

After RNA quantification using the Nanodrop 2000 (Thermo Fisher Scientific, USA), 1 g of total RNA was reverse transcribed in a total volume of 20 l using the PrimeScript RT reagent kit (Takara Bio, Tokyo, Japan)