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New York, NY: Raven Press, Ltd., 1994., p
Natural products such as Huangkui capsule and quercetin offer multi-target, holistic benefits with minimal side effects, though standardization and large-scale clinical validation are needed
Acknowledgments This work was supported by grants from the National Natural Science Foundation of China (Grant Numbers: 81572224, 81572230, 81772438, and 81671102), The National Key Research and Development Program of China, Stem Cell and Translational Research (2017YFA0105104), Science and Technology Planning Project of Guangdong Province, China (Grant Numbers: 2016A020213003, 2016B030230002, and 2017A040406007), Science and Technology Planning Project of Guangzhou, China (Grant Number: 2016201604030036), and the National Key Clinical Department, National Key Discipline, Guangdong Provincial Key Laboratory for Diagnosis and Treatment of Major Neurological Disease (Grant Numbers: 2014B030301035).
The GST-fusion protein was adsorbed onto a glutathione-sepharose resin (Cytiva), recovered by cleavage with 3 C protease and purified by size exclusion chromatography using a Superdex 200 column
