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Death versus survival: functional interaction between the apoptotic and stress-inducible heat shock protein pathways

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jejuni (Cj) to a CFE reaction to glycosylate a cell-free synthesized protein containing a glycosylation tag

In one example, the T7 RNA polymerase-promoter system is used and is induced from the lac operon in BL21 (DE3)-based strains to circumvent the need to separately purify and supplement T7 RNA polymerase into CFE reactions