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The major contributor of superoxide radicals (O 2 ) is NADPH oxidase, which is synthesized during cellular respiration by reducing one-electron oxygen molecules with electrons delivered by NADPH

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For other ratios, researchers reconstitute separate vials and combine in solution

In yet another aspect, there is provided a method of screening for an agent that sensitizes cancer cells to cytotoxic T cell-mediated killing and/or immune checkpoint therapy, the method comprising a) contacting cancer cells with cytotoxic T cells and/or immune checkpoint therapy in the presence of monocytes and/or macrophages contacted with i) at least one agent that reduces the copy number, amount, and/or activity of at least one target listed in table 1 and/or ii) at least one agent that increases the copy number, amount, and/or activity of at least one target listed in table 2