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2.6 Evaluation of intracellular oxidative stress levels by H 2 DCFDA oxidation assay Oxidative stress levels in lymphoblasts were evaluated through indirect measuring of 2,7-dichlorodihydrofluorescein diacetate (H 2 DCFDA) oxidation by flow cytometry, based on the assumption that DCF fluorescence is proportional to the concentration of reactive redox species present in the cytoplasm[56]