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2.4 Sample preparation For LCQTOF-MS/MS analysis, 1 mg of powder or homogenized tablet was dissolved or extracted using 5 mL of a 1:1:1 (v/v/v) mixture of water/methanol/acetonitrile, assisted by ultrasonication for 10 min, and afterwards filtered by a Whatman 0.2 m pore size polytetrafluoroethylene (PTFE) filter medium (GE Healthcare, Chicago, IL, United States)

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It also offers protection from harmful substances entering the bloodstream from the compromised GI tract because of IBS further reducing the incidence of inflammation and other IBS symptoms

For the full background on this four-compound grouping, see our KLOW stack research overview