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Cells were manually scraped, spun at a 100 g for 5 min, the pellets washed with PBS and then homogenized in a Tris salt buffer, transferred to ultracentrifuge tubes and spun at 163, 200 g and 4 C for 30 min to extract the soluble fraction

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Growth hormone promotes skeletal muscle cell fusion independent of insulin-like growth factor 1 up-regulation

[28:40] Natalie Kitroeff He's saying basically that he's willing to sacrifice sexual enjoyment for the goal of looking good, becoming more beautiful

Li et al., 2025)