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doi:10.5455/jpma.40329

Homogenised tissue was filtered through a 40 m sieve (Corning, Massachusetts, United States), centrifuged at 500 g for 5 min at 4 C to pellet the nuclei and resuspended in buffer supplemented with 1% BSA and 0.2 U/l RNAase inhibitor (Takara Bio, Kusatsu, Japan)

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Sperandio S, Poksay K, de Belle I, Lafuente MJ, Liu B, Nasir J, et al

Upon external stimulation, signaling molecules bind to their receptors, leading to the activation of the IKK complex (via phosphorylation of IKK), which phosphorylates IB, causing its degradation and dissociation from NF-B dimers

their role in periparturient dairy cattle health regulation