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Several techniques have been developed for the evaluation of the BBB such as the in vivo brain uptake index (BUI) method (88), in situ brain perfusion (89), cerebral microdialysis technique (90, 91), and cell uptake studies in the in vitro models of the BBB (92)

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The methods utilized for compound identification and quantification are detailed below: L- and D-lactate Fifty microliter of sample were dried and then derivatized by adding 50 l of (+)- O,O -diacetyl-L-tartaric anhydride solution (97%) (DATAN) (SigmaAldrich, United States) [100 mg ml -1 , where DATAN was dissolved in dichloromethane:acetic acid (4: 1, by volume)]

The siRNAs (siNC, siGGCT162, siGGCT299) were synthesized in GenePharma Company (Shanghai, China)

1992;29(1):6568 Lenzi A, Picardo M, Gandini L, Dondero F

Blood 106(11):521A-521A, 2005