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J Psychiatr Res (2016) 82:10918

Role of plasma membrane transport in hepatic glutamine metabolism

Cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 7.5, 500 mM NaCl, 0.5 mM TCEP, 5% glycerol, 1.0% Triton X-100, 0.1 mM PLP, 2 mg/ml lysozyme) and lysed by sonication

As well, the authors found differences in the total number of laid eggs at the end of the experiment, the hens fed with 5% supplementation of SB had higher number of eggs

Immunoprecipitation (IP) and western blotting (WB) were performed as previously described 33

Within 24 to 48 hours : For others, it may take a day or two to notice a difference