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Characterizing microglia activation: a spatial statistics approach to maximize information extraction

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Funding Statement This work was supported by Japan Science and Technology Agency (JST) CREST Grant Number JPMJCR2024 (20348438 to T.A.), Grant-in-Aid for Scientific Research on Innovative Areas(A) Sulfur biology (21H05263 and 21H05258 to T.A.), International Leading Research (23K20040 to T.A.), Scientific Research (S) (24H00063 to T.A.), from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and Japan Agency for Medical Research and Development (AMED) to T.A

S., Depratt, C., & Reyes, F

We attached recombinant CspZ proteins to VLPs by adding an engineered C-terminal cysteine to CspZ and linking it to surface-exposed lysine amino groups of VLPs using SMPH (Succinimidyl 6-((beta-maleimidopropionamido)hexanoate)) cross-linker (Figure 1A)

Gold (Zhao et al., 2022), silver (Gupta et al., 2019) represent the most common inorganic core decorated with polymeric backbones (Bilal et al., 2020)