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(PubMed) Gao L, Mao Q, Cao J, Wang Y, Zhou X, Fan L

Protein concentrations in lysates were determined by Protein Assay Dye Reagent (BioRad #5000006), diluted in 2x Laemmli sample buffer (BioRad Cat#1610737) with fresh -mercaptoethanol (BioRad #1610710), and heated at 95C for 5 minutes

When the gut lining is exposed to severe irritantssuch as bacterial endotoxins (LPS) or excessive ammonia produced by dysbiotic bacteriathe epithelial cells release zonulin, which signals the tight junctions to open, increasing intestinal permeability

Zhang, S.A

Astrocytes were excluded from the analysis due to their morphology and their high fluorescence levels 68

pylori may contribute to the development of cholangiocarcinoma caused by O