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The resulting sections were mildly fixed with 2% paraformaldehyde (VWR Life Science) for 10 min, permeabilized by incubation in 0.05% Triton X-100 for 10 min and blocked with 5% BSA for 1 h at room temperature

The patient harboured the following genetic point mutations: KRAS (G61H), SMAD4 (Asn107Lys), BRCA2 (Arg2034Cys), and PRSS (Cys171Tyr)

Moreover, the mechanisms may be related to the upregulation of the cellular NAD/SIRT1 pathway and the recovery of the PI3K/AKT insulin signaling pathway [69]

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H 2 S Reactivity H 2 S has strong nucleophilic properties that allow it to react with electrophilic species, whereas sulfur is a good reductant and will react with oxidants