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Biochemical analyses Transfected N2a cells were lysed with ice-cold Lysis buffer (20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM MgCl 2 , 2 mM EDTA, 1% Triton X-100, 0.1% SDS, and 10% glycerol) supplemented with a protease inhibitor cocktail (Roche)

With once-daily dosing, steady-state peak concentrations are established within approximately one week

A meta-analytic summary of systemic GSH indicates variable doseresponse and inconsistent biomarker effects, complicating mechanistic attribution

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Bonciolini V

10.7326/0003-4819-121-12-199412150-00009 50 FukudaS.NojimaJ.MotokiY.YamagutiK.NakatomiY.OkawaN.et al