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& Venhoff, N

10.1016/j.sajb.2024.05.011 211 NiZ.LiuN.YuY.BiC.ChenQ.QuY

3D cell culture models Most in vitro studies of ECD rely on 2D cell models where a monolayer of ECs is grown on tissue culture polystyrene (TCPs) plates [237]

One of the central regulatory pathway in redox balance is the Kelch-like ECH-Associating protein 1 (Keap1)nuclear factor erythroid 2 related factor 2 (Nrf2)antioxidant response element (ARE) pathway, which upregulates biosynthesis of antioxidant proteins, including antioxidant enzymes such as heme oxygenase-1 (HO-1), glutathione S -transferase, NAD(P)H:quinone oxidoreductase 1 (NQO1), glutathione reductase (GR), Trx-R, CAT, and SOD, but also nonenzymatic antioxidant proteins, e.g., Trx or anti-apoptotic proteins from Bcl-2 family 64

Recent, critical research has demonstrated that in the TME, particularly under conditions of glucose deprivation, high lactate also drives the lactylation of nonhistone protein NMNAT1