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Skeletal muscle single fiber enzymatic isolation and morphology EDL muscles were digested in Tyrode solution (without FCS) containing 3 mg/mL collagenase type IVA (Sigma Aldrich,United States), gently bubbled with oxygen, and maintained at 37C

JAMA Internal Medicine , 177 , 930938

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Dysbiosis, characterized by reduced diversity and changes in the composition of the gut microbiome, is associated with overweight and obesity (5)

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