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RNA extraction and Real-time PCR For gene expression analyses, total RNA was extracted from cells or liver tissues using Trizol and Tissue Lyser for 3 m in while for cells the RNeasy Plus Mini Kit (Qiagen) was used

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This result is consistent with earlier reports on the barrier and homeostasis functions of cell membranes in the stress response of O.oeni , which is well-known (Grandvalet et al., 2008)

USA 110 , 81828187 (2013)

Similarly, a cross-sectional study by Shea et al