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Milani GP, Alberti I, Abodi M, Lakoumentas J, Konstantinou GN, Papadopoulos NG, et al

OG also ameliorated non-alcoholic fatty liver disease (NAFLD), as evidenced by reduced hepatic lipid accumulation and normalized adipose tissue metabolism

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Calcium influx was measured using flow cytometry for 5 min on a BD FACSymphony A5 flow cytometer, and calcium flux was quantified as the ratio of Indo-1 emission at 400 nm (bound Ca 2+ ) to 475 nm (free Ca 2+ )

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Prism v.8.0 (GraphPad Software, San Diego, California, United States) software was then utilized for comprehensive data analysis, precise curve fitting, accurate plotting, and ultimately, the determination of the half-maximal inhibitory concentration ( IC 50 ) value representing the inhibitory effect of CPI2 on CTSS