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While these model systems provide valuable insights into liver function and drug metabolism, they fail to mimic the intricate three-dimensional (3D) architecture, cellular heterogeneity, and zonation observed in the native liver

Among these, gutka, naswar, paan, khaini, and zarda were locally prepared and available in loose packaging, while paan masala was tested using the most common commercial brand (Rajni Brand)

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Under caspase-8 inhibition, RIPK1/RIPK3 phosphorylation initiates necrosome formation and MLKL activation, triggering membrane breakdown and the release of pro-inflammatory damage-associated molecular patterns (DAMPs) (Fritsch et al., 2019

The solvent should be introduced slowly down the inner glass wall of the vial, allowing the liquid to naturally wick into the peptide cake via capillary action

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