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Development of stable isotope dilution assays for the analysis of natural forms of vitamin B12 in meat

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In the first step, the hydroxyl oxygen atom of Ser657 attacks the P1-carbonyl carbon of the peptide substrate due to the action of the catalytic triad His86 as a general base to extract the proton of the Ser657 OH group (Fig

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SH groups activate many enzyme systems involved in protein synthesis as well as fat and carbohydrate metabolism