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Total phospholipids appear to vary through lactation, with some studies reporting a decrease over time (58, 81, 8386), whereas in one study the total milk phospholipids concentration was reported to not vary across lactation (82)

The reactions were performed in a 25 l volume of KAPA SYBR FAST qPCR kit (Kapa biosystems, USA) with 10 picomole of forward and reverse primer from each gene ( Table 1 ) using Mx-3000P spectro flourometric thermocycler (Stratagene, USA)

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PMID 34894966 Editor information Editors and Affiliations Rights and permissions Copyright information 2023 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd

ISHIDA, T., Suzuki, T., Hirashima, S., Mizutani, K., Yoshida, A., Ando, I., Ikeda, S., Adachi, T., and Hashimoto, H