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Interestingly, MeCbl blocks GSDME-mediated pyroptosis, but barely has any effect on apoptosis when Gsdme genetic deficiency cells were challenged with apoptotic stimulation

The process is straightforward but requires attention to detail

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In particular, external (cytoplasmic) Cl concentrations exhibit a biphasic effect on glutamate transport with maximal activity in the presence of 430 mM Cl , depending on glutamate concentrations used for the transport assay (40 M and 5 mM glutamate, respectively