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A new, sterile syringe and needle must be used for every single puncture of any vial

An in vitro study (67), where human ectopic endometriotic cells were cultured with curcumin, showed a significant inhibition of TNF--induced secretion of IL-6, IL-8 and MCP-1 , known actors in the development of endometriosis

For many experiment, we also used 10 nM of ligand for stimulation, which is a more physiological level than the 100 nM used in many previous experiments

And, pretreatment with Moringa oleifera leaf extract (400 mg/kg/day for 14 days) produced anxiolytic effects in both FST and tail suspension test (TST) in the hepatic encephalopathy mouse model (139)