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Your choice of binding buffer will influence the binding capacity of the beads , so if you are capturing substantially less than 8 mg of protein per mL of resin, and your protein isnt really big, then it is worth considering if your binding buffer is limiting the capture efficiency of the beads

[4] Understanding how each agent works is essential before considering their combined use

doi: 10.1016/0891-5849(95)00011-l

These can be shared electronically or in print, with a clinician available to explain any findings and guide your next steps

Armstrong et al., 2018)