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The plates were washed three times with PBST (0.05% Tween 20 in PBS), and 100 L of tetramethyl benzidine (TMB) solution (ThermoFisher, Waltham, MA, USA) were added to each well and incubated for 5 min

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Additionally, both low and high doses of taurine (100 and 400 mg/kg via oral gavage) over nine weeks increased GSH in brain homogenates of mice exposed to D-galactose (22) and improved cognitive deficits in the aging model used in that study

The GEM of an organism is an in silico stoichiometric model curated to capture its metabolic inventory from available knowledge such as genome annotation mapped to gene-associated reactions and metabolites

Another case involved an anaphylactic shock after a hydroxocobalamin injection