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Layer 2/3 fibers were stimulated by placing a tungsten bipolar electrode in the IL cortex

Identification of genes involved in the decrease of opacity using microarrays To identify the genes associated with the development of lens opacity induced by galactose and the effect of glutamate on opacity reduction, we cultured 2 samples (Control) for 4 and 6 days without galactose, 3 samples (Galactose) were cultured for 6 days in a medium containing galactose to induce opacity, and 2 samples (Glutamate) were cultured in a medium containing galactose for 3 days to induce opacity, followed by the addition of glutamate and an additional 3 days of culture for microarray analysis

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DNA/protein damage markers Oxidative stress-induced DNA and protein damage in MASLD are important indicators of disease progression [130]

Timing Optional Marker ESR Why it matters Adds a slower-moving inflammation marker that may be useful when symptoms are chronic

DNA were extracted from blood and tumor tissue of the same patient (12 from Ile/Ile, 12 from Ile/Val and 6 from Val/Val) and digested with nuclease P1 and calf intestinal phosphatase (Sigma, USA) and denatured