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Sci Transl Med (2018) 10(458):eaas9563

Effects of Hericium erinaceus on nerve growth factor (NGF) secretion in astrocyte cells

BALF supernatants were collected for biochemical analyses, and cell pellets were resuspended in 500 l of PBS

However, canavanine in the feedstuffs can be inactivated by different treatment methods, primarily soaking, acid treatment, alkali treatment, or heat treatment (Sadeghi et al., 2004)