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O.VandenabeeleP.Vanden BergheT

While Glutathione cannot officially be classified as an antioxidant under FDA guidelines, its indirect role in neutralizing free radicals and its pivotal contributions to cellular defense have earned it this informal title

The eluted protein was concentrated, and buffer exchanged into 50 mM NaH 2 PO 4 , 300 mM NaCl, 10 % v/v glycerol, pH 8.0 using Vivaspin 5 5000 Da MWCO spin columns (Cytiva)