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If symptoms havent improved, it strongly suggests B12 was not the root cause, and further medical investigation is needed

Rapamycin significantly inhibits the activity of arthritis and improves immune function in RA mice [117, 118]

Western blot analysis In order to further verify the above results, we employed western blotting to analyze the expression levels of IL-6 in nerve tissues from both the control group and the DP-treated group

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Cells were washed three times with ice-cold PBS and lysed in lysis buffer (40 mM Tris-HCl (pH 8.0), 1% Triton X-100, 1% NP-40, 10% glycerol, 0.15 M NaCl, 2 mM EDTA, 1 mM phenylmethanesulfonyl fluoride, 1 Complete protease inhibitor cocktail)