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Every 50 oocytes were cultured in 100 l mini-drops of MEM+ containing 20% FBS covered with mineral oil in an incubator at 37.0 C, 5% O 2 , and 5% CO 2 in a humidified atmosphere

In response to TNF-, certain substrates including RIP1, TRAF2, and cIAP1 are modified with K63-linked chains by enzymatic activity of cIAP1, which recruits LUBAC and induces a phosphorylation cascade that eventually activates NF-Bmediated gene induction 46

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The hypertrophy of the alveolar cells, the increased permeability and the gene expression variation were recorded with the developed lung-on-chip, while the transwell-mediated coculture failed to recapitulate what occurs in vivo