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Another study reported that PINK1 phosphorylated MFN2 at Thr111 and Ser442 to induce MFN2 association with parkin and cause ubiquitination and subsequent degradation of MFN2 ( ATP13A2 ATP13A2 encodes the lysosomal multiple transmembrane P5B-type cation-transporting ATPase ATP13A2

After incubation, nuclei were counterstained with DAPI and washed three times with PBS

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However, the testis-specific mammalian TrxR3 protein, also called thioredoxin-glutathione reductase or TGR, as well as the Sec-lacking TrxR protein in , does reduce GSSG (Gromer et al., 2003