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Following primary antibody incubation, cells were treated with goat anti-rabbit IgG H&L Alexa Fluor 488 (ab150077, Abcam), donkey anti-goat IgG H&L Alexa Fluor 647 (ab150131, Abcam) and DAPI (Invitrogen) for 1 hour in the dark at room temperature

To induce mitochondrial mistranslation, we used the peptide mimetic actinonin (ACT) resulting in the accumulation of mistranslated, membrane-inserted mitochondrial proteins causing subsequent membrane potential loss 16,17,18,19,20,21

Supplementing Glycine and N-Acetylcysteine (GlyNAC) in Older Adults Improves Glutathione Deficiency, Oxidative Stress, Mitochondrial Dysfunction, Inflammation, Physical Function, and Aging Hallmarks: A Randomized Clinical Trial Liang, H., et al

Colon cancer chemopreventive activities of pomegranate ellagitannins and urolithins

An auto-sampler with a volume of 20 L was used