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The organic layer was reconstituted in chloroform:methanol (2:1 v/v, 200 l and diluted 1:10 with 2:1:1 isopropanol:acetonitrile:water, with the addition of an internal standard mix composed of isotopically labelled compounds from major lipid classes

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It plays a crucial role in neutralizing harmful free radicals, detoxifying the body, and supporting a healthy immune system